【摘要】 目的采用薄膜分散-超声法制备磁性颗粒(bacterial magnetic particles,BMPs)脂质体,考察BMPs浓度、超声功率和超声时间等因素对BMPs磁性脂质体粒径的影响。方法薄膜分散-超声法制备BMPs脂质体,调节BMPs浓度、超声功率和超声时间等因素,激光散射粒度仪测定磁性脂质体粒径。结果以薄膜分散-超声法制备的BMPs脂质体,BMPs浓度在(20～60)μg/mL时,磁性脂质体粒径基本稳定,平均94.9 nm;BMPs浓度在(60～100)μg/mL时,磁性脂质体粒径随着BMPs浓度增加而有增大的趋势。超声功率的增加或超声有效时间增大时,磁性脂质体的平均粒径有减小趋势,当超声功率为300 W、有效超声时间为100 s时,粒径出现最小值;但其后都存在转折,随着超声功率的再增加或超声有效时间再增大时,平均粒径反而出现增大现象。结论薄膜分散-超声法制备BMPs脂质体,通过控制BMPs浓度、超声功率和超声时间等因素,可以对磁性脂质体粒径进行调节。更多还原

【Abstract】 Objective Magnetic liposomes from bacterial magnetic particles(BMPs) were prepared by film dispersion-ultrasonic method,and the effects of BMPs’ concentration,ultrasonic power and ultrasonic time on the size of BMPs’ magnetic liposome were investigated.Method The BMPs concentration,ultrasonic power,ultrasonic time and other factors were measured,and the magnetic liposome sizes were tested by Laser scattering particle size analyzer.Results When the BMPs concentration was 20~60 μg/mL,the magnetic liposome size was stable with 94.9nm average diameter;When the BMPs’ concentration was 60~100 μg/mL,the size of magnetic liposome had a positive correlation with the concentration,but had a negative correlation with the ultrasonic power and the ultrasound effective time until reached to the minimum when the ultrasonic power was 300 W and the effective ultrasound time was 100 s.Beyond this power and ultrasound time,the size increased.Conclusion The sizes of BMPs’ magnetic liposomes can be regulated by the concentration of BMPs,ultrasonic power,ultrasonic time.更多还原