【摘要】 目的建立测定橘红化痰消积合剂中柚皮苷含量的高效液相色谱法。方法色谱柱:Phenomenex-C18(4.6mm×250mm,5μm),流动相:甲醇-醋酸-水(38∶0.5∶62);检测波长:283 nm;流速:1.0 mL/min;柱温:40℃。结果柚皮苷在85.6-856μg之间,线性关系良好,加样回收率99.07%,RSD=1.04%(n=6)。结论该方法操作简便、准确,具有很好的重现性和稳定性,可以用于橘红化痰消积合剂的质量控制。更多还原

【Abstract】 Objective To select the method for Determination of Naringin in Juhonghuatanxiaoji Oral Solution by HPLC.Methods The column employed was Phenomenex-C18 Column(4.6mm×250mm,5 μm);The mobile phase was methanol-acetic acid-water(38∶0.5∶62);The detection was effected at 283 nm;the flow rate was 1.0 mL/min and column temperature was 40 ℃.Results The linear range of Naringin was 85.6-856 μg(r=0.9999),the mean recovery was 99.07%,RSD=1.04%(n=6).Conclution This method is simple,rapid and accurate with good reproducibility.It is suitable for the quality control of Juhonghuatanxiaoji Oral Solution.更多还原