【摘要】 为研究Ms5基因在红麻(Hibiscus canabius L.)花药中的转录特征,以L23A不育系为试材,基于已知的红麻Ms5基因5′端cDNA序列,采用3′RACE和RT-PCR技术对该基因全长cDNA序列进行克隆。序列比对分析结果表明,红麻花药组织中同时存在Ms5基因2种不同的转录本,其长度分别为972和1 105bp,依次命名为HcMs5-α和HcMs5-β。这2种转录本均包含了完整且一致的编码序列,其编码序列(CDS)全长为858bp,预测编码的蛋白质含有285个氨基酸残基,分子质量为32.4ku,理论等电点为7.62;两者5′端前972bp序列完全一致,但3′端的非编码区(UTR)长度有所不同,HcMs5-β在第972bp以后比HcMs5-α多出133bp的序列,具有选择性转录终止的特征。这种转录终止信号的可选择性可能与该基因转录后水平的调控有关。更多还原

【Abstract】 The full mRNA of kenaf Ms5 gene was tried to clone with RT-PCR and 3′RACE methods based on the known partial 5′-end cDNA sequence.The sequencing results showed that two transcripted cDNAs of Ms5 gene were cloned from the anther of kenaf.The length of two transcripts named as HcMs5-α and HcMs5-β were 972 bp and 1 105 bp,respectively.The structure analysis showed that both of them contained an 858 bp ORF,encoding a protein of 285 amino acids with predicted molecular weight of 32.4 ku and a isoelectric point of 7.62.The sequencing results showed that 5′-end 972 bp sequence of the two transcripts was identical,but HcMs5-β possessed an additional 133 bp sequence at 3′-end after 972 bp compared with HcMs5-α.The alternative termination of transcription in the 3′UTR of kenaf Ms5 maybe related with the post transcriptional regulation of the gene.It is the first report about the multiple transcripts of Ms5 gene in higher plant.更多还原