【摘要】 为探讨苏云金芽胞杆菌高温诱导产黑色素机制,利用含mini-Tn10转座子载体pIC333构建苏云金芽胞杆菌油松亚种4CA1,随机插入突变体库,以寻找相关调控基因。通过高温诱导筛选得到1株在42℃产黑色素减弱的菌株;克隆得到mini-Tn10中断基因的部分序列,通过测序及同源性比对分析,确定中断基因为超氧化物歧化酶基因sodA2。更多还原

【Abstract】 Bacillus thuringiensis subsp.yosoo 4CA1 can not produce melanin at 28℃,while it may possess high yield of melanin when induced at 42℃.In order to study the mechanism of melanin production induced by high temperature,a random insertional mutant library of B.thuringiensis subsp.yosoo 4CA1 was constructed with plasmid pIC333 containing the mini-Tn10 transposon.Compared with the wild-type strain 4CA1,a mutant of producing melanintion weakly at 42℃ was obtained.Then the flanking sequence of mini-Tn10 was cloned and sequenced.The results showed that the gene interrupted by mini-Tn10 was sodA2.更多还原