【摘要】 目的探讨壳寡糖促进糖尿病模型大鼠难愈感染创面愈合作用。方法制备糖尿病大鼠创面模型。实验组以5%壳寡糖涂抹创面,每天1次,共21d,动态观察局部细菌总量变化及创面愈合指标,并检测创面组织中的TGF-β1浓度的变化。结果与结论5%壳寡糖治疗组第7、14、21天创面愈合率均显著高于对照组,创面在伤后3周的愈合率达到100%,实验第3、7天壳寡糖治疗组总菌量明显低于糖尿病对照组,壳寡糖治疗组创面组织中TGF-β1浓度在创伤后3、7、14d与糖尿病对照组相比浓度均明显增高,结果表明5%壳寡糖能促进糖尿病大鼠难愈创面的愈合,此疗效与壳寡糖的抗菌作用及提高创面组织的TGF-β1浓度有关。更多还原

【Abstract】 Objective To investigate the effects of chitosan on the infected wound healing in diabetic rats.Methods The Wistar rats were used to make the models of the diabetes,a round skin wound was established on the dorsal backs of rat.And then the rats were divided into groups: the test group was treated with 5% chitosan group.The total amount of bacteria,the healing rate of wound and the concentration of TGF-β1 in the wounds were observed and compared with the model group.Results and Conclussion At the 7,14 and 21 day,the healing rate of 5% chitosan group was higher than that of diabetes control group,and reached to 100% in the 21 day;The total amount of bacteria of the 5% chitosan group was remarkably less than that of diabetes control group at the 3 and 7 day;the concentration of TGF-β1 in 5% chitosan group was significantly higher than that in diabetic model group at the 3,7 and 14 day.Results shows that 5% chitosan can promote the healing of the infected wound in diabetic rats,the excellent antibacterial functions of chitosan and increased amount of TGF-β1 in the wound would be probably considered an important reason.更多还原