【摘要】 为研究波纹唇鱼(Cheilinus undulatus)线粒体D-loop序列遗传多样性,作者采用聚合酶链式反应和克隆技术对海波纹唇鱼群体(n=25)的mtDNA D-loop序列进行克隆和测序,获得长度大约为1 400 bp的产物。用ClustalX软件进行排序比较,将结果导入MEGA5.0,结果显示出在这25尾个体中,共检测出66个变异位点,包括0个碱基缺失,4个碱基插入,59转换位点,2颠换位点及1个转换和颠换同时存在的位点。并用MEGA5.0软件构建该群25尾个体的NJ和UPGMA系统树。由DNASP软件计算出该波纹唇鱼群体的多态位点数(S)为62,核苷酸多样性(Pi)为0.00606,平均核苷酸差异数为6.907。结果表明波纹唇鱼群体的mtDNA-loop基因个体差异程度不明显。更多还原

【Abstract】 The aim of this paper was to study the variations in the mtDNA D-loop sequences of Cheilinus undulatus populations.Twenty-five individuals of C.undulatus were collected from the offshore area of Hainan province.One thousand and four hundreds bp nucleotide sequences of mtDNA D-loop region were obtained using PCR and cloning techniques.66 variation sites including 0 deletion,4 inserts,59 transition sites,2 transversion sites and 1 transition-transversion sites were observed using ClustalX and MEGA5.0 softwares.The pairwise genetic distances of these 25 individuals were calculated using the MEGA5.0 software and based on that,the NJ phylogenetic trees was contracted for these fish populations.Analysis of this fish populations using the DNASP software showed that they had a polymorphic sites(S) of 62,a nucleotide diversity(Pi) of 0.00606 and an average number of nucleotide differences(K) of 6.907,respectively.Overall,there were no significant variations between mtDNA D-loops of C.undulatus populations.更多还原