【摘要】 用壳聚糖添加量为0.00%(D0)、0.25%(D1)、0.50%(D2)、1.00%(D3)和2.00%(D4)的实验饲料投喂虹鳟幼鱼50d,研究其对虹鳟幼鱼生长性能、体组成及非特异性免疫的影响。结果显示,各实验组增重率(WGR)、特定生长率(SGR)显著高于对照组(P<0.05),饲料系数(FCR)、脏体比(VSI)呈显著降低趋势(P<0.05),而肝体比(HSI)、脾体比(SSI)、肥满度(CF)及存活率(SR)无明显差异(P>0.05)。全鱼、肌肉及肝脏粗脂肪含量显著降低(P<0.05)。D3组和D4组碱性磷酸酶(ALP)活性显著低于其它各组(P<0.05);酸性磷酸酶(ACP)活性显著降低(P<0.05),溶菌酶(LZM)活性则显著升高(P<0.05);D4组总抗氧化能力(T-AOC)显著高于对照组及D1组(P<0.05);超氧化物歧化酶(SOD)活力差异不显著(P>0.05)。在本实验条件下,饲料中添加壳聚糖可显著提高虹鳟幼鱼生长性能、增强非特异性免疫能力,以增重率及非特异性免疫为综合评价指标,虹鳟饲料中壳聚糖的适宜添加量为0.50%。更多还原

【Abstract】 Rainbow trout was cultured widespread all around the world, but the healthy of rainbow trout was generally inhibited by high-density culture. A 50-d feeding trial was carried out to investigate the effects of dietary chitosan on growth performance, body composition and non-specific immunity of juvenile Oncorhynchus mykiss [(98.26±0.25)g]. Five isonitrogenous and isoenergetic diets were formulated by adding 0% (D0, control group), 0.25% (D1), 0.50% (D2), 1.00% (D3) and 2.00% (D4) chitosan to the basal diet, in which casein and fish meal were used as the protein source and fish oil as the lipid source, respectively. Results showed that: weight gain rate (WGR) and specific growth rate (SGR) increased whereas feed conversion ratio (FCR) decreased significantly (P<0.05) with chitosan addition No significant differences were found among chitosan-supplemented groups (P>0.05). Viscerosomatic index (VSI) decreased (P<0.05) with dietary chitosan supplementation (P<0.05). However, there were no statistical differences in hepatosomatic index (HSI), spleen-somatic index (SSI), condition factor (CF) or survival ratio (SR) (P>0.05). Lipid content in whole fish, muscle and liver were decreased obviously by dietary chitosan (P<0.05) , while no change existed in moisture and protein contents (P>0.05). Alkaline phosphatase (ALP) activity in serum of D3 and D4 groups was decreased significantly (P<0.05) and there were no differences among other groups (P>0.05). Serum acid phosphatase (ACP) activity decreased as dietary chitosan supple-mentation level increased (P<0.05), while lysozyme (LZM) activity was increased (P<0.05). Total antioxidant capacity (T-AOC) of D4 group was significantly higher than that of the control and D1 group (P<0.05) and no differences were found among other groups (P>0.05). Superoxide dismutase (SOD) was not affected by dietary chitosan (P>0.05).更多还原