【摘要】 稀土纳米氧化物和稀土化合物的生物效应已引起人们的广泛关注.利用3-(4,5-二甲基噻唑-2)-2,5-二苯基四氮唑溴盐(MTT),流式细胞术法和激光共聚焦显微镜初步探讨了纳米Eu2O3和Eu3+对体外培养的人肝癌细胞HepG2生长的影响.结果发现,较低浓度的纳米Eu2O3对细胞生长没有明显影响,浓度达到600μg mL-1作用癌细胞仅24 h,细胞就停止分裂,表现为细胞被阻滞在S期,大量细胞坏死.利用激光共聚焦显微镜观察到纳米Eu2O3能进入活的HepG2细胞中.而Eu3+则在较低含量,即≤100μmol L-1时能较弱地抑制癌细胞的生长,细胞被阻滞在G0/G1期,并诱导细胞发生凋亡.更多还原

【Abstract】 The biological effects of rare earth elements and rare earth nanoparticles have aroused an extensive interest during the past few years.In vitro culture conditions,we reported the impact of nano Eu2O3 and Eu3+ on the HepG2 cells using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide(MTT),flow cytometry and laser scanning confocal microscopy.The results suggested that the nano Eu2O3 at low concentrations does not make an obvious impact on cell growth whereas it can suspend cell division,and the percentage of cells in G2/M phase increase gradually and lead to necrosis at higher concentrations(≥600 μg mL-1).In addition,nano Eu2O3 can be internalized by live HepG2 cells.Eu3 + at low concentrations(≤100 μmol L-1) can slightly inhibit the growth of HepG2 cells by arresting the ratio of G0/G1phase cells and triggerring apoptosis.更多还原