【摘要】 在玻碳电极上电沉积石墨烯/壳聚糖/碳纳米管复合膜,通过戊二醛连接抗体,建立了新型电流型甲胎蛋白传感器,通过循环伏安法和交流阻抗考察了其电化学特性。在优化的实验条件下,传感器的峰电流随着检测溶液中甲胎蛋白(AFP)浓度的增大而增大,并在0.05～100ng/mL浓度范围内呈现线性关系,回归方程为△I=0.51c+0.68(ng/mL);检测限为0.02ng/mL(R=0.9990)。该免疫传感器具有制作简单、灵敏度较高、重现性好、线性范围宽等优点,可用于临床上对AFP的检测。更多还原

【Abstract】 An electrochemical immunosensor for the detection of alpha fetal protein(AFP) has been developed based on graphene/chitosan/carbon nanotube modified glassy carbon electrode.The modified electrode was characterized by cyclic voltammetry and impedance spectroscopy.Under the optimum conditions,the detection is based on the peak current change of the modified electrode before and after the antigen-antibody reaction.The linear range is 0.05~100 ng/mL.The linear regression equation is △I=0.51c+0.68(ng/mL).The detection limit is 0.02 ng/mL(R=0.9990).The sensor has good reproducibility,selectivity and stability,and was applied to determine human serum with satisfied result.更多还原