【摘要】 目的研究微小核糖核酸-21(miR-21)反义寡核苷酸(AS-miR-21)对人舌鳞癌生长的抑制作用。方法采用稳定转染pGL6荧光素酶报告基因质粒的舌鳞癌细胞Tca8113-luc接种裸鼠,建立移植瘤动物模型,瘤体内注射AS-miR-21,应用活体成像和TUNEL等实验技术进行AS-miR-21对人舌鳞癌细胞系生长抑制的体内研究。结果通过转染pGL6荧光素酶报告基因质粒构建了稳定表达荧光素酶活性的Tca8113-luc细胞株。裸鼠皮下荷瘤模型成瘤率高,肿瘤生长稳定。在瘤体内注射AS-miR-21后肿瘤生长减慢,活体成像中光子数偏低,肿瘤标本中坏死灶少见,细胞核变小,染色变浅,异型性减低,新生血管数减少。肿瘤组织中miR-21表达明显下降,凋亡指数升高。结论肿瘤内注射AS-miR-21可以降低舌鳞癌细胞中miR-21的表达,促进舌鳞癌肿瘤细胞凋亡,抑制肿瘤的生长。更多还原

【Abstract】 Objective To detect antisense-micro ribonucleic acid-21 oligonucleotide(AS-miR-21)’s inhibiting effect to tongue squamous cell carcinoma.Methods Living image and TUNEL experiments were performed,based upon the xenograft animal models set up by introduction of Tca8113-luc cells which were stably transfected with pGL6 lucife-rase report gene plasmid into nude mice,while the tumors were injected with AS-miR-21.Results Tca8113-luc cell line which steadily expressed luciferase activity was constructed by transfecting pGL6 report gene plasmid.The subcu-taneous tumor formation rate was much higher in nude mice introduced with the cells,and the tumors grew well.After injection of AS-miR-21 into mice tumors,it was obviously viewed that tumors grew slower,the volume of the tumors was smaller,the photon number in live body imaging was getting less,the necrosis in the tumor specimens was rare,cell nuclei was getting smaller,dyeing color was lighter,heteromorphism and new vessels were decreased,micro ribo-nucleic acid-21 expression in tumor cells was considerably lower,and apoptotic index was increased.Conclusion All the results indicate that the injection of AS-miR-21 can inhibit growth of tongue squamous cell carcinoma in nude mice model,and effectively promote cell apoptosis of tongue squamous cell carcinoma.更多还原