【摘要】 目的研究微小核糖核酸-21(miR-21)对人舌鳞状细胞癌细胞系生物学特性的影响。方法将2’O-Me修饰的正义和反义miR-21寡核苷酸序列通过脂质体转染技术转染到舌鳞状细胞癌细胞Tca8113及其高转移株内,采用实时荧光定量聚合酶链式反应检测转染后miR-21在细胞中的表达变化,通过甲基噻唑基四唑(MTT)法、流式细胞术、AnnexinⅤ细胞早期凋亡检测、划痕实验及Transwell实验分别检测细胞增殖能力、细胞周期、细胞早期凋亡情况、细胞迁移及侵袭能力的变化。结果靶向miR-21的反义寡核苷酸可以有效地抑制Tca8113及其高转移株细胞的增殖,促进细胞凋亡,并抑制细胞的迁移和侵袭能力。结论细胞内转染反义miR-21寡核苷酸序列后,可以降低舌鳞状细胞癌细胞中miR-21的表达,并对舌鳞状细胞癌细胞的生物学行为产生影响。更多还原

【Abstract】 Objective To detect the biological influence to human tongue squamous cell carcinoma(TSCC) cells of micro ribonucleic acid-21(miR-21).Methods Referring to mature miR-21 sequence,the sense and antisense oligonu-cleotide(sense-miR-21 and AS-miR-21) modified by 2’O-Me were designed to transfect into TSCC cells(Tca8113 and high metastasis cells) by liposome transfection technology,in order to establish an in vitro TSCC cell model.The expression changes of miR-21 in the transfected cells were detected with real-time fluorescence quantitative polyme-rase chain reaction(real-time PCR).The changes of cell proliferation,cell cycle,cell early apoptosis,cell migration and invasion capabilities were detected respectively by the technologies of methyl thiazolyl tetrazolium(MTT),flow cytometry, Annexin Ⅴ cell early apoptosis assay,scratch assay and Transwell assay,to check AS-miR-21’s effect on the biological characteristics of human TSCC cell lines.Results For the TSCC cells,the antisense oligonucleotide of targeting miR-21 could effectively inhibit cell proliferation,promoted cell apoptosis,and inhibited the capability of cell’s migration and invasion.Conclusion The expressions of miR-21 decrease after AS-miR-21 transfected into TSCC cells,and miR-21 can affect biological behavior of TSCC cells.更多还原