【摘要】 目的探讨细胞内液渗透压对三叉神经节神经元(TRGN)上的电压门控钠离子通道(VGSCs)的生物力学影响。方法 TRGN细胞从SD乳鼠分离出来之后,运用膜片钳全细胞记录的方法对VGSCs电流进行记录和分析,通过调节电极内液的组成成分来控制细胞内液渗透压;再与正常渗透压相对比,探讨低渗(260 mOsm)和高渗(350 mOsm)对通道激活和失活动力学的影响。结果与对照组相比较,细胞内液低渗刺激可以影响VGSCs电流的激活和失活特性,包括V0.5、失活曲线(G-V曲线)特征均有明显的统计学意义(P<0.05);然而对于细胞内高渗刺激,仅VGSCs电流的失活特性有一定改变,包括失活速率和k值。结论三叉神经细胞上VGSCs的动力学参数可以通过细胞内液低渗和高渗进行调节,并可表现为不同的特征。更多还原

【Abstract】 Objective To evaluate the biomechanical effects of intracellular changes on the voltage-gated sodium channels(VGSCs) on trigeminal ganglion neuron(TRGN).Methods TRGN cells were acutely isolated from the neona-tal SD rats.The voltage-dependent currents of the VGSCs on these neurons were elicited and analyzed by whole-cell patch-clamp recordings and the intracellular anisotonicity stimuli was established by adjusting the content of pipette solution.The effects of hypo-(260 mOsm) and hypertonic(350 mOsm) osmolarity on the activation and inactivation kinetics of VGSCs on TRGN were evaluated,compared with the normal intracellular environment.Results The results demonstrated that intracellular hypotonic stimuli could influence both the activation and inactivation characteristics of VGSCs currents,including the membrane potential at half inactivation(V0.5) of the G-V and inactivation curves had obvious statistics significance(P<0.05) between hypotonicity(260 mOsm) and isotonicity(306 mOsm).However,only inac-tivation properties changed under intracellular hypertonic effects,including inactivation rate and k value.Conclusion It suggests that the kinetics of VGSCs on TRGN can be modulated both by intracellular hypo-and hypertonic with different characteristics.更多还原