【摘要】 表面增强拉曼散射(surface-enhanced Raman scattering,SERS)技术以其独特的谱带窄、灵敏度高、抗光漂白、原位和无损等优势,在疾病诊断和生物分析领域得到了越来越广泛的应用。本文介绍了近几年来应用于生物大分子、病原微生物、细胞和活体检测分析中的最新SERS技术,并分别从标记与非标记的角度对其进行了阐述,总结了SERS标记检测生物大分子的基本识别模式,简述了检测低浓度病原微生物的SERS技术,着重评述了SERS检测技术在细胞和活体研究中的应用,并对基于SERS的疾病诊断和生物分析技术的发展趋势进行了初步展望。更多还原

【Abstract】 Surface-enhanced Raman scattering(SERS) technique has experienced unprecedented growth in recent years,driven in large part by its numerous merits including sharp bandwidth,high sensitivity,anti-photobleaching as well as the ability of in-situ and noninvasive analysis for diagnosis and bioassay.Recent advances in SERS-based detection and analysis techniques toward the targets of biomacromolecules,pathogenic microorganisms,cells and living organisms,with an emphasis on labeled or label-free techniques are discussed in the review.General methods are summaried in basic recognition modes for different objects in SERS detection.Details are listed to highlight the strategies to increase sensitivity in the detection of pathogenic microorganisms.We also provide insights into the latest applications of SERS techniques in vivo and present the prospects of SERS techniques in the last part of the article.更多还原