【摘要】 为进一步了解棉铃虫体色突变体的成色和调控机理,利用SDS-聚丙烯酰胺凝胶电泳(SDS-PAGE)技术、基质辅助激光解吸电离串联飞行时间质谱技术(MALDI-TOF-TOF/MS)和生物信息学技术,对棉铃虫显性、隐性体色突变体与野生型成虫的蛋白质组成进行了比较分析。研究发现:显性体色突变体与野生型之间差异不明显,而隐性体色突变体和野生型之间存在一些明显差异,选取其中比较明显的3条差异条带进行质谱分析,发现一些与能量代谢、细胞骨架相关的蛋白。结果表明,这2类蛋白可能与棉铃虫体色突变性状的发生和调控相关。更多还原

【Abstract】 To gain an in-depth understanding of the fineness and regulation mechanism of body color mutant of Helicoverpa armigera Hbner,the protein composition differences between adult of dominant mutant,recessive mutant and wild type were studied using the SDS-PAGE combined with MALDI-TOF-TOF/MS and bioinformatic analysis.The results indicated that the protein composition of the dominant mutant and wild type had little difference.However,there were obvious differences between the recessive mutant and wild-type.Three specific strips were chosen for mass spectrometry and bioinformatic analysis,and two types of proteins related to energy metabolism and cytoskeleton were identified.These findings suggested that the two types of proteins may be associated with occurrence and regulation of body color mutant traits of H.armigera.更多还原