【摘要】 建立了气相色谱-电子捕获检测器测定牛奶中五氯苯酚残留量的检测方法.样品经丙酮提取,乙酸酐衍生化,衍生物采用气相色谱-电子捕获检测器进行检测.结果表明,五氯苯酚衍生物响应信号与其质量浓度在5.00～500.00μg/L范围内线性关系良好,相关系数r2为0.999;在5、10和50μg/kg添加水平下,牛奶中待测物平均回收率分别为77.6%、87.2%和86.3%,相对标准偏差(RSD)小于9.6%;检测限为2μg/kg,定量限为5μg/kg.方法适合于牛奶中五氯苯酚残留检测.更多还原

【Abstract】 A method was established for determining residue of pentachlorophenol in milk with gas chromatography(GC)-electron capture detector(ECD).The sample was extracted with acetone and derived with acetic anhydride.The derivative was determined by gas chromatography with electron capture detector.Good accuracy,precision and sensitivity were obtained with the method.The calibration curves of the derivative of pentachlorophenol were linear between the peak areas and the concentrations in the range of 5.00-500.00 μg/L with correlation coefficients(r2) of 0.999.The average recoveries of pentachlorophenol were 77.6%,87.2% and 86.3% respectively at three spiked concentration of levels of 5,10 and 50 μg/kg,with relative standard deviations(RSDs) less than 9.6%.The limit of detection(LOD) for the derivative of pentachlorophenol was 2 μg/kg and the limit of quantitation(LOQ) was found to be 5 μg/kg.The study revealed that the method was suitable for the determination of pentachlomphenol in milk.更多还原