To investigate the relationship between Ghrelin and the expression of BCL-2 and BAX and the mechanism of ghrelin in sheep maturation process of sheep oocytes,500 ng/mL of Ghrelin was added to the mature culture medium of sheep oocytes.Then,the mRNA expressions of BCL-2 and BAX in the oocytes at 0,8,16 and 24 h were evaluated by the fluorescent real time quantification PCR,respectively.The results showed that the relative BCL-2 mRNA level of sheep oocytes increased significantly(P<0.05) after being cultured ...
【基金】
国家自然科学基金项目(30660128)
【更新日期】
2012-03-31
【分类号】
S826
【正文快照】
Effect of Ghrelin on mRNA expression of BCL-2 and BAX during in vitromaturation of sheep oocytesBAI Rui-xia1,2,CAO Gui-fang1,MI Yan1,TA Na1(1.College of Veterinary Medicine,Inner Mongolia Agricultural University,Hohhot 010018,China;Ghrelin是1999年日本科学