【摘要】 目的比较不同地区大气PM2.5对血管内皮细胞的毒性,并探讨其作用机制。方法采集广州、东莞、深圳和肇庆4个城市的PM2.5。试验细胞为人脐静脉内皮细胞。将PM2.5分别以10、50、100、200、300μg/ml剂量染毒,作用于细胞24 h,测定细胞NO释放量、SOD活力、LDH漏出量及细胞存活率(MTT试验)。以肇庆作为对照,观察PM2.5对各指标的影响。经剂量与指标回归分析,以斜率评价不同城市PM2.5的效应大小。结果各城市PM2.5致细胞NO释放量和LDH漏出量随剂量增加而升高,SOD活力和细胞存活率随剂量升高而降低(P<0.05)。广州和深圳PM2.5在高剂量时致NO释放量明显高于肇庆(P<0.05)。广州、东莞和深圳PM2.5致SOD活力降低的程度在各剂量组均强于肇庆(P<0.05)。深圳PM2.5致LDH释放量明显高于肇庆,广州、东莞在高剂量时高于肇庆(P<0.05)。广州、深圳PM2.5致细胞死亡率高于肇庆(P<0.05)。回归分析表明,广州PM2.5致LDH漏出和细胞死亡的毒性效应最高,深圳PM2.5对NO释放和SOD活力降低的毒性效应最高,肇庆PM2.5对NO、SOD、LDH的细胞毒性均处于最低水平。NO释放量、LDH漏出量与细胞存活率呈负相关,SOD活力与细胞存活率呈正相关(P<0.01)。结论不同城市PM2.5对血管内皮细胞的毒性表现不同。NO、SOD、LDH与细胞存活率有关联,氧化应激损伤可能是其作用机制之一。更多还原

【Abstract】 Objective To know the toxic effects of particulate matter from different cities on human umbilical vein endothelial cells and its mechanism.Methods Airborne particulate matter PM2.5 was collected in four cities of south China in 2009.The cells(umbilical vein endothelial cells) were exposed to the PM2.5 at the dose of 10,50,100,200 and,300 μg/ml respectively for 24 h,and then NO production,LDH release,SOD activity and viability of the cells(MTT) were measured.Using the PM2.5 from Zhaoqing city as the control,the effects were compared.Results The NO production and LDH release increased,and SOD MTT decreased significantly with increase in dose(one way ANOVA,P<0.05),which was observed in every city samples.Compared between cities,the PM in Guangzhou and Shenzhen produced more NO at higher dose than that in Zhaoqing(P<0.05).The PM in Guangzhou,Dongguan and Shenzhen inhibited SOD more serious compared with the control(P<0.05).The PM in Shenzhen resulted in more LDH release significantly than that in Zhaoqing(P<0.05),which was found in the Guangzhou and Dongguan PM only at higher dose and the cell survival rate in group of Guangzhou and Shenzhen were significantly lower(P<0.05).The regression analysis showed that the Guangzhou PM had the highest toxicity in the aspect of LDH leakage and cell death,the Shenzhen particle was toxic at the highest level in producing NO and inhibiting SOD activity,and in contrast the toxicity of Zhaoqing PM was at the lowest in NO,SOD and LDH.In addition,the NO and LDH were correlated with MTT negatively,and SOD with MTT positively(P<0.01).Conclusion The PM2.5 in haze whether showes more severe toxicity.The toxicity of PM from different areas is not homogeneous.The indicator of NO,SOD and LDH is correlated closely to cell death,oxidative stress might be a mechanism of cardiovascular toxicity caused by airborne particles.更多还原