【摘要】 目的研究自然杀伤(NK)细胞活性变化与膝关节骨性关节炎(knee osteoarthrosis,K-OA)的关系。方法选择K-OA患者50例,作为K-OA组,无关节炎病史及自身免疫病者41例作为对照组,补体裂解法分离K-OA组与对照组外周血NK细胞,酶免疫标记技术测定两种不同NK细胞亚群比例,MTT法检测NK细胞杀伤活性。结果与对照组比较,K-OA组CD16-CD5+6比例减少,而CD1+6CD5+6比例上升(P<0.05),NK细胞杀伤活性增强。结论 K-OA的发生与具有杀伤活性的CD1+6CD5+6NK细胞亚群比例上升,杀伤活性增强有关。更多还原

【Abstract】 Objective To investigate the correlation between the activity of nature killer(NK) cell in peripheral blood and knee osteoarthrosis(K-OA).Methods 50 patients with K-OA were enrolled in K-OA group,and 41 patients without arthritis history and autoimmune diseases were enrolled in control group.The NK cells were separated by means of complement lysis method,then the proportion of two kinds of NK cell was detected,and the killing activity of NK cell was detected by MTT.Results As compared with control group,the proportion of CD16-CD56+ was decreased,however,the proportion of CD16+CD56+ was increased(P<0.05),and the activity of NK cell was enhanced.Conclusion The pathogenesis of K-OA is correlated with the increase of the proportion of CD16+ CD56+ NK cell subgroup and with the increase of the killing activity of NK cells.更多还原