【摘要】 目的建立胰岛素抵抗心肌细胞模型。方法原代培养2～3d SD大鼠心肌细胞分为对照组、葡萄糖组（glucose,G）、胰岛素组（insulin,Ins）、葡萄糖+胰岛素组（C+Ins）。在干预24h和48h后评价细胞的胰岛素敏感性,检测脂联素表达。结果干预48h后,Ins组、G+Ins组心肌细胞³H-D-葡萄糖掺入率分别为（12.46±1.11）nmol·mg^-1·h^-1、（10.61±1.14）nmol·mg^-1·h^-1,均较对照组的（14.06±0.43）nmol·mg^-1·h^-1显著下降,差异有统计学意义（P<0.05）。干预48h后,Ins组、G+Ins组心肌细胞脂联素mRNA表达分别为0.35±0.04、0.38±0.04,均较对照组的0.77±0.08显著下降,差异有统计学意义（P<0.05）。对照组、G组、Ins组、G+Ins组³H-D-葡萄糖掺入率与心肌细胞脂联素mRNA表达量均呈显著正相关（r=0.92、0.82、0.89、0.86,P均<0.05）。结论应用高胰岛素诱导法、高葡萄糖+高胰岛素共同诱导法均可建立胰岛素抵抗心肌细胞模型;胰岛素抵抗下调心肌细胞脂联素表达水平;胰岛素抵抗心肌细胞脂联素表达水平与胰岛素抵抗呈正相关。更多还原

【Abstract】 Objective To establish insulin resistance cardiocytes model.Methods Cardiocytes were cultured for 24h and 48h in vitro and were divided into four groups:control group（control）,glucose group（G）,insulin group（Ins）,glucose and insulin group（G + Ins）.The adiponectin（APN） expression in those groups was tested and the insulin sensitivity was evaluated by ³H - D - glucose mixing experiment.Results H - D - glucose mixing rate in Ins group（12.46±1.11） nmol ? mg^-1 ? h^-1 and G + Ins group（10.61±1.14） nmol ? mg ? h were all lower than control group（14.06±0.43） nmol ? mg^-1 ? h^-1（P<0.05）.APN expression in Ins group 0.35±0.04,G + Ins group 0.38±0.04,were all lower than control group 0.77±0.08 after 48h（P < 0.05）.³H - D - glucose mixing rate in four groups was positively related to APN expression（P < 0.05 ）.Conclusion The insulin resistance cardiocytes model can be established by high insulin or high glucose + insulin.Insulin resistance down - regulates cardiocytes adiponectin expression.Insulin resistance is positively related to APN expression.更多还原