【摘要】 该研究利用纯化后的流行性乙型脑炎病毒(Japanese encephalitis virus,JEV)E-Ⅲ抗原域蛋白,将其作为包被抗原,建立了间接ELISA检测方法。试验的最佳反应条件为:最佳抗原包被浓度为17.5μg/mL,血清抗体稀释度为1∶4,酶标二抗稀释度为1∶5 000,封闭液和稀释液用含0.4%BSA的PBST。采用该方法对127份临床样品进行检测,结果显示,与商品试剂盒检测结果比较符合率达到90.00%。因此,该研究建立的流行性乙型脑炎间接ELISA检测方法具有良好的稳定性和可重复性,并具有较高的特异性和敏感性。更多还原

【Abstract】 The Ⅲ antigenic region of Japanese encephalitis virus’s E protein was used as a coating antigen to establish an indirect ELISA assay.The factors affecting the experiments were optimized as using 17.5 μg/mL E protein as coating antigen,1∶4 dilution of testing serum and 1∶5 000 dilution of HRP-labeled goat anti-swine IgG,PBST containing 0.4% BSA as the blocking buffer and diluting buffer.127 serum samples were detected by this assay,which showed 90.00% agreement with ELISA kit.The results showed that the indirect ELISA had good reliability,speciality and sensitivity for clinic serum samples.更多还原