【摘要】 为建立适合沙棘(Hippophae rhamnoides L.)的SRAP-PCR反应体系,对影响SRAP-PCR的Mg2+浓度、Taq DNA聚合酶浓度、引物浓度、dNTPs浓度、模板DNA浓度进行了优化。优化后的反应体系为Mg2+3.0 mmol/L、Taq DNA聚合酶2U/25μL、引物0.8μmol/L、dNTPs 0.25 mmol/L、模板DNA 30 ng/25μL,反应总体系25μL。利用此体系从30对引物组合中筛选出17对适合沙棘SRAP-PCR的引物,有助于沙棘的分子标记辅助育种研究。更多还原

【Abstract】 The concentrations of Mg2+,Taq DNA polymerase,primers,dNTPs,template DNA affecting the SRAP-PCR reaction were optimized to establish the SRAP-PCR system for sea buckthorn(Hippophae rhamnoides L.).The optimum 25 μL reaction system contained 3.0 mmol/L Mg2+,2 U/25 μL Taq DNA polymerase,0.8 μmol/L primers,0.25 mmol/L dNTPs and 30 ng/25 μL DNA templates.17 out of 30 primer pair combinations were screened out using the optimized amplification system,which would support the molecular marker assisted breeding of sea buckthorn.更多还原