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甲醇浸提法提取荠菜黄酮工艺优化
Optimization of the Extraction of Flavonoids from Capsella bursa-pastoris with Methanol
【摘要】 以荠菜为原料,研究了甲醇浓度、浸提温度、浸提时间、料液配比等单因素对荠菜中黄酮类化合物浸提效果的影响。在单因素试验的基础上,通过二次回归正交旋转试验得到了最佳提取工艺:甲醇体积分数为0.125,浸提时间为2.9 h,料液质量比为1∶32.7。在最佳浸提条件下得到荠菜黄酮类物质提取量为每克干样中芦丁当量29.33 mg。
【Abstract】 In this paper,effects of methanol concentration,temperature,extracting time and ratio of solid to liquid on extracting flavonoids from Capsella bursa-pastoris were studied.By single-factor and second order regression for rotatable orthogonal designs,the optimum extracting conditions were as following:time was 2.9 h,solid-to-liquid ratio 1∶ 32.7,methanol concentration 0.125.The yield of flavonoids was 29.33 mg equivalent rutin/g dry sample at the optimum conditions.
【关键词】 黄酮类化合物;
提取;
二次回归正交旋转试验;
【Key words】 flavonoids; extracting; second order regression for rotatable orthogonal design;
【Key words】 flavonoids; extracting; second order regression for rotatable orthogonal design;
- 【文献出处】 河北科技师范学院学报 ,Journal of Hebei Normal University of Science & Technology , 编辑部邮箱 ,2012年01期
- 【分类号】TQ461
- 【被引频次】3
- 【下载频次】221