【摘要】 目的初步探讨儿童刺激性全唾液中蛋白成分与乳牙患龋状况的关系。方法单纯随机抽样法选取4~5岁、性别匹配的高龋(龋、失、补指数≥5,高龋组)和无龋(龋、失、补指数=0,无龋组)儿童各40例。吐取法收集刺激性全唾液,双金鸡纳酸法测定总蛋白含量。十二烷基硫酸钠-聚丙烯酰氨凝胶电泳定量分析各蛋白成分。结果高龋组儿童唾液中总蛋白含量低于无龋组(P<0.01)。平均每个样品分离出10条可分析条带,两组比较,10000、28000、38000、56000、77000蛋白百分含量高龋组均高于无龋组;14500蛋白百分含量高龋组与无龋组比较差异无统计学意义(P=0.137)。结论患龋程度不同的儿童唾液中,分子量为10000、28000、38000、56000、77000的蛋白含量有差异,可能与乳牙龋易感性不同有关。更多还原

【Abstract】 Objective To investigate the correlation between the concentrations of proteins in stimulated whole saliva and the dental caries susceptibilities of deciduous teeth of children. Methods Based on simple random sampling, 40 children of 4~5 years old with high dmft (high-caries group, dmft≥5) and 40 children in same age (free-caries group, dmft = 0) were selected. Stimulated whole saliva from each child was collected by direct spitting method. The level of salivary total proteins was measured by means of bicinchoninic/BCA. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) was performed to separate the salivary proteins. Results The concentrations of proteins in saliva was significantly lower in high-caries group than free-caries group (P<0.01). Ten straps in average were isolated from children’s stimulated saliva using 15% SDS-PAGE. about The concentrations of 10 000, 28 000, 38 000, 56 000 and 77 000 proteins was higher in high-caries group than free-caries group, whereas, no statistically significant difference was found in the concentration of 14 500 Da protein (P = 0.137). Conclusion Statistically significant difference was found in 10 000, 28 000, 38 000, 56 000, 77 000 protein in the saliva of children with different degrees of dental caries, which might be correlated to the different dental caries susceptibilities.更多还原