【摘要】 目的:研究脐血来源高增殖潜能内皮祖细胞支持脐血造血干/祖细胞体外扩增的能力。方法:利用第3代的HPP-EPC联合细胞因子培养脐血造血干/祖细胞,分因子组、非接触培养组和接触培养组。体外培养脐血CD34~+细胞10 d后,从细胞总数,CD34~+细胞数,各系造血集落形成细胞数等比较各组的扩增效率。结果:接触组细胞总数扩增倍数(44.6±8.7)倍和非接触组(39.5±7.3)倍均高于因子组(24.8±5.6)倍,P<0.01。接触组CD34~+细胞数扩增倍数(8.8±2.1)倍高于因子组(2.9±0.6)倍和非接触培养组(3.3±0.6)倍,P<0.01。接触组集落扩增倍数和非接触组均分别高于因子组,P<0.05,且接触组各造血集落扩增数均分别高于非接触组,各P<0.05。接触组扩增后CD34~+CD38~-细胞(10.8%±2.7%)明显高于因子组(4.1%±0.9%)和非接触培养组(3.8%±0.8%),P<0.01。结论:脐血来源高增殖潜能内皮祖细胞能支持脐血CD34~+细胞体外扩增。更多还原

【Abstract】 Objective:To investigate whether human cord blood derived high proliferative potential endothelial progenitor cells(HPP-EPC) can support the expansion of hematopoietic progenitor cells from human umbilical cord blood.Methods:Passage 3 HPP-EPCs were used to expand CD34~+ cells from fresh umbilical cord blood in 3 groups:cytokines group as control,non contact group and contact group.After 10 d culture,total cell number,CD34~+cell number,colonies of hematopoietic progenitors were counted to analyze the expansion effects in each group.Results:After 10 d expansion,the expansion folds of total cell number in both contact group(44.6±8.7) and non contact group(39.5±7.3) were higher than in cytokine group(24.8±5.6,both P<0.01).The expansion fold of CD34~+ was higher in contact group(8.8±2.1) than in non contact group (3.3±0.6) or cytokine group(2.9±0.6)(both P<0.01).The expansion folds of colonies in contact group and in non contact group were higher than those in cytokine group(all P<0.05).And the expansion folds of colony number of hematopoietic progenitors in contact group were higher than those in non contact group(all P< 0.05).The rate of CD34~+ CD38 ~ cells after expansion ex vivo in contact group(10.8%±2.7%) was higher than that in cytokine group(4.1%±0.9%) and non contact group(3.8%±0.8%).Conclusion:Human umbilical cord blood derived HPP-EPC can support the expansion of CD34~+ hematopoietic stem/progenitor cells from human umbilical cord blood.更多还原