【摘要】 本文应用超高效液相色谱法,Acquity UPLC HSS T3(2.1×100 mm×1.8μm)柱,0.1%三氟乙酸-乙腈流动相,梯度洗脱,紫外和荧光串联检测器,可同时测定保健食品中6种水溶性维生素,其中包括Vc、B3、B1、B6、B11、B2等,该法线性范围宽、呈直线回归、相关性好、检测限低、分析速度快,其测定结果与国标法相吻合。更多还原

【Abstract】 Water-soluble vitamins were determiend by Ultra-performance liquid chromatography(UPLC) equipping ultraviolet detector and fluorescence detector in series.Samples were separated by a Acquity UPLC HSS T3(2.1×100 mm×1.8 μm) column using 0.1% trifluoroacetic acid-acetonitrile as mobile phase in gradient elution mode.Six water-soluble vitamin components in health foods were simultaneously determined,including ascorbic acid(Vc),nicotinamide(B3),thiamine(B1),pyridoxine(B6),folic acid(B11),riboflavin(B2),etc.This method was enabled to achieve wide linear range,well correlation,low detection limit and fast analysis.The results were in accordance with those wtih GB methods.更多还原