【摘要】 目的探讨肾血管性高血压大鼠左心室肥厚和蛋白激酶B(PKB)之间的关系。方法将20只雄性SD大鼠随机分为手术组和假手术组,每组10只。手术组行二肾一夹手术,致大鼠肾血管性高血压,假手术组行假手术。术前及术后每4周测鼠尾动脉血压1次。术后12周处死动物,采用Western blot方法检测心肌组织中蛋白激酶B(PKB)表达。结果与假手术组相比,手术组术后4周、8周、12周血压明显升高(P<0.05);手术组左心室质量指数(LVMI)、左心室壁厚度(LVWT)、室间隔厚度(IVST)均明显高于假手术组(P均<0.05);手术组的PKB蛋白表达明显高于假手术组(P<0.05)。结论 PKB途径可能是肾血管性高血压致左心室肥厚相关信号转导途径之一。更多还原

【Abstract】 Objective To explore the relationship between left ventricular hypertrophy(LVH) and protein kinase B(PKB) in rats with renovascular hypertension.Methods Twenty male SD rats were randomly divided into two groups:operation group and sham-operation group.There were 10 rats in each group.The two-kidney-one-clip(2K1C) operation was performed to establish the left ventricular hypertrophy model with renovascular hypertension in operation group.Sham-operation group underwent sham operation.Blood pressure of rats was measured once before operation and every 4 weeks after operation,respectively.Animals were killed 12 weeks after operation.Protein expression of PKB in myocardial tissues was detected by Western blot assay.Results Compared with sham-operation group,blood pressures in operation group at the 4th,8th,12th week after operation increased significantly(P<0.05);Left ventricular mass index(LVMI),left ventricular wall thickness(LVWT),interventricular septal thickness(IVST) in operation group were significantly higher than those in sham-operation group(P<0.05);Protein expression of PKB in operation group was significantly higher than that in sham-operation group(P<0.05).Conclusion The PKB pathway might be one of signal transduction pathways which is related with LVH induced by renovascular hypertension.更多还原