【摘要】 目的探讨鼻咽癌组织中血管内皮生长因子-C(VEGF-C)及淋巴管内皮透明质酸受体-1(LYVE-1)的表达与患者生存率的关系。方法采用免疫组织化学二步法,检测56例鼻咽癌标本和15例正常鼻咽黏膜标本中VEGF-C的表达情况,并通过淋巴管内皮细胞特异性标记物———LYVE-1染色,计数鼻咽癌组织中淋巴管密度(LVD)值,分析鼻咽癌组织中VEGF-C与LVD与患者生存率的关系。结果 (1)56例鼻咽癌组织和15例正常黏膜组VEGF-C的阳性表达率分别为83.9%(47/56)和26.7%(4/15),差异有统计学意义(P<0.01)。鼻咽癌组LVD值为(20.00±4.37),正常黏膜组为(10.12±1.79),差异有统计学意义(P<0.01)。(2)VEGF-C、LVD与生存率的关系:56例患者3年累积生存率为64.0%,经Log-rank检验结果显示VEGF-C高表达、高LVD 3年累积生存率均低于VEGF-C低表达、低LVD患者差异均有统计学意义(P<0.05)。VEGF-C、LVD与患者生存率呈负相关(P<0.05)。结论鼻咽癌组织中VEGF-C与LVD的表达明显高于正常鼻咽黏膜上皮;VEGF-C高表达、高LVD鼻咽癌患者生存率较低,预后较差。更多还原

【Abstract】 Objective To explore the relationship between expression of vascular endothelial growth factor-C(VEGF-C),lymphatic vessel endothelial hyaluronic acid receptor 1(LYVE-1) in nasopharyngeal carcinoma and patients′ survival.Methods Immunohistochemistry(IHC) was used to examine the expression of VEGF-C in 56 cases of nasopharyngeal carcinoma and 15 cases of normal mucous membrane of nasopharynx,and lymphatic vessel density(LVD) in nasopharyngeal carcinoma was counted by LYVE-1 staining which was specific marker for lymphatic vessel endothelial cell,and the relationships among VEGF-C,LVD and patents′ survival were analyzed.Results(1)The expression of VEGF-C in 56 cases of nasopharyngeal carcinoma and 15 cases of normal mucous membrane of nasopharynx were 83.92%(47/56) and 26.7%(4/15) respectively,and there was significant difference between these two groups(P<0.01).LVD of nasopharyngeal carcinoma group was 20.00±4.37,LVD of normal mucous membrane of nasopharynx group was 10.12±1.79,and there was significant difference between these two groups(P<0.01).(2)The relationship between VEGF-C,LVD and patients′ survival:the 3-year cumulative survival of 56 cases of nasopharyngeal carcinoma was 64.0%,the Log-rank test showed that the 3-year cumulative survival of the patients with high expression of VEGF-C and high LVD was lower than that of the patients negatively with low expression of VEGF-C and low LVD(P<0.05).VEGF-C and LVD were correlated with the patients′ survival(P<0.05).Conclusion The expression of VEGF-C and LVD in nasopharyngeal carcinoma is higher than that in normal mucous membrane of nasopharynx.Patients with high expression of VEGF-C and high LVD have low survival and poor prognosis.更多还原