【摘要】 目的:建立正常人表皮黑素细胞体外培养体系,观察不同浓度白介素-8对体外培养正常人黑素细胞的细胞增殖及黑素合成的影响。方法:采用四甲基偶氮唑盐(MTT)法测定不同浓度白介素-8对黑素细胞增值的影响,NaOH裂解法测定黑素生成量,流式细胞仪检测黑素细胞的凋亡率。结果:白介素-8对黑素细胞活力有增强作用,能使细胞增殖能力增加,黑素合成增加,但对黑素细胞的凋亡率无显著性影响。结论:白介素-8对体外培养的黑素细胞增殖及黑素生成都有增强作用。更多还原

【Abstract】 Objective: To establish the culture system of human normal melanocytes in vitro,and to study the effects of interleukin-8 at different concentrations on the proliferation of human melanocytes and melanin synthesis.Methods: MTT method and NaOH lysis method were employed to measure the effects of interleukin-8 at different concentrations on the proliferation of human melanocytes and melanin respectively.Apoptosis rates of melanocytes were detected with flow cytometer.Results: Interleukin-8 promotes melanocyte proliferation and melanin synthesis.Conclusions: Interleukin-8 enhances melanocyte proliferation and melanin synthesis.更多还原