【摘要】 目的:观察95%二氯吡啶酸原药对鼠伤寒沙门菌回复突变试验(Ames试验)菌株基因突变和中国地鼠肺(CHL)细胞染色体畸变的影响,初步探讨其遗传毒性,为该农药的毒理学安全性评价提供基础数据。方法:采用Ames试验及CHL细胞染色体畸变试验在直接作用或代谢活化条件下,分别设立试验剂量组和阴性、阳性对照组,37℃培养检测。结果:95%二氯吡啶酸原药在直接作用或代谢活化条件下,对鼠伤寒沙门菌TA97、TA98、TA100、TA102各剂量组回复突变菌落数均未超过阴性对照组回变菌落数的2倍;CHL细胞染色体畸变试验显示各剂量组与阴性对照组比较差异均无统计学意义(P>0.05)。结论:在本试验条件下,95%二氯吡啶酸原药无诱发鼠伤寒沙门菌基因突变和CHL细胞染色体畸变作用。更多还原

【Abstract】 Objective: To observe the effects of clopyralid at concertration of 95% on gene mutation of bacteria strains used in Ames test and chromosomal aberration of Chinese hamster lung(CHL) cell,and to explore genotoxicity of clopyralid to obtain basic data for its toxicological safety evaluation.Methods: Ames test and chromosomal aberration test in CHL were employed to examine genotoxicity of clopyralid.Resluts: By direct action or under metabolic activation,the numbers of reverse mutation colonies of TA97,TA98,TA100 and TA102 of Salmonella typhimurium induced by each dosage of 95% clopyralid were less than twice that of negative control group.There was no significant difference in chromosomal aberration rate of CHL cell between each clopyralid dosage group and control group(P>0.05).Conclusions: Under this experimental condition,95% clopyralid could not cause gene mutation of S.typhimurium or chromosomal aberration of CHL cell.更多还原