【摘要】 目的:探讨正常人外周血T淋巴细胞亚群端粒酶活性表达情况。方法:采用端粒重复序列扩增—聚合酶链反应—酶联免疫吸附测定(TRAP-PCR-ELISA)方法半定量检测CD4+T淋巴细胞和CD8+T淋巴细胞的端粒酶活性。结果:CD4+T淋巴细胞端粒酶阳性率43.8%(7/16),CD8+T淋巴细胞端粒酶阳性率37.5%(6/16)。结论:正常人外周血CD4+T淋巴细胞和CD8+T淋巴细胞可表达端粒酶活性。更多还原

【Abstract】 Objective: To explore the characteristics of telomerase activity of human peripheral blood T lymphocytes.Methods: Telomerase activity of T lymphocytes was determined by polymerase chain reaction-based enzyme immunoassay(PCR-ELISA) using telomerase repeat amp lification protocol(TRAP).Results: The positive rates of telomerase activity of human peripheral blood CD4+T and CD8+T lymphocytes were 43.8%(7/16) and 37.5%(6/16) respectively.Conclusion: Human peripheral blood CD4+T and CD8+T lymphocytes express telomerase activity.更多还原