【摘要】 目的:观察前成骨样细胞(MC3T3-E1细胞)分化过程中17β-雌二醇对胰岛素受体底物-1(insulin receptor substrate-1,IRS-1)的影响。方法:应用10mmol/Lβ-甘油磷酸钠和50mg/L抗坏血酸诱导小鼠MC3T3-E1细胞分化成熟,分别在0,6,12,18,24,30d收集细胞。17β-雌二醇干预,用半定量RT-PCR和免疫印迹法检测干预组和对照组细胞IRS-1mRNA和蛋白的表达,比较两组细胞IRS-1表达量的变化。结果:在MC3T3-E1细胞分化成熟过程中,IRS-1 mRNA和蛋白的表达在MC3T3-E1细胞分化成熟过程中逐渐增高。结论:IRS-1可能在17β-雌二醇诱导的骨转换过程中起着重要作用。更多还原

【Abstract】 Objective:To observe the effect of estradiol on the expression profile of insulin receptor substrate-1(IRS-1) during murine preosteoblastic MC3T3-E1 cells differentiation.Methods: MC3T3-E1 cell was cultured and underwent intervention of 17β-estradial.Cells were collected respectively in days 0,6,12,18,24 and 30.Semi-quantitative RT-PCR was used to measure the expression of mRNA of IRS-1.The expression of the proteins of IRS-1 was detected by Western blotting.Results: IRS-1 mRNA and protein levels gradually increased during differentiation of MC3T3-E1 cells.Estradiol increased IRS-1 mRNA and protein expressions in the whole time course.Conclusion: IRS-1 may play an important role in the process of estradiol inducing bone turnover.更多还原