【摘要】 【目的】通过测定广西流行狂犬病毒糖蛋白(G)基因的全序列,了解广西狂犬病的流行趋势和遗传变异规律,为制定广西狂犬病的防控策略提供科学依据。【方法】2000年10月～2007年10月,从广西14个市采集1569份动物脑组织,经RT-PCR检测及小白鼠脑内接种试验分离狂犬病毒,然后对狂犬病毒G基因进行测序分析。【结果】从广西各地共分离获得26株狂犬病毒野毒株,其中犬23份,牛、猪、猫各1份。狂犬病毒G基因核苷酸全长2067bp,开放阅读框1575bp,编码524个氨基酸。根据G基因核苷酸同源性及遗传进化树分析结果,可将广西流行的狂犬病毒株分成3个群,Ⅰ群15株,其核苷酸同源性为97.7%～99.9%;Ⅱ群10株,其核苷酸同源性为98.0%～99.9%;Ⅲ群只有1株。通过氨基酸变异分析,了解了26株野毒株G基因编码蛋白的氨基酸突变位点。【结论】广西存在3个群的狂犬病毒野毒株,且以Ⅰ群和Ⅱ群流行为主,3个群的氨基酸变异均呈现明显的特异性。更多还原

【Abstract】 [Objective]In order to understand the epidemic trend and genetic variation of rabies disease in Guangxi and to provide relevant prevention and control strategies, the glycoprotein (G) gene sequences, isolated from Guangxi rabies virus strains, were studied and analyzed. [Method]From Oct. 2000 to Oct. 2007, 1569 samples of animal brain tissues collected from 14 cities of Guangxi were used to isolate and detect rabies virus by RT-PCR and Mouse Intracerebral Inoculation Test, then they were sequenced according to procedure. [Result]The results showed that 26 wild strains of rabies were collected from different areas of Guangxi; out of the strains, 23 belonged to dogs, 1 belonged to cattle,1 belonged to swine, and 1 belonged to cat. The whole length of the nucleotide of rabies virus G gene was 2067 bp, and the open reading frame was 1575 bp, which encoded 524 amino acid. According to homology and evolutionary tree analysis results, the isolated Guangxi rabies virus G genes were divided into three gene-groups, namely groups Ⅰ (15 strains), Ⅱ(10 strains), and Ⅲ(1 strains). The nucleotide homology rates of groups I and Ⅱ were 97.7-99.9% and 98.0-99.9%, respectively. Amino acid mutation sites of glycoprotein encoded by rabies virus G genes in 26 wild strains were obtained by analyzing the amino acid variation. [Conclusion]There were three groups of rabies virus virulent strains, of which groups Ⅰand Ⅱ were more common. The amino acid variations in each of the three groups were significantly specified.更多还原