【摘要】 根据日本晴cab4基因序列(GenBank:AK104499.1)设计引物,用RT-PCR的方法从籼稻9311中克隆了叶绿素a/b结合蛋白基因的全长cDNA,命名为cab-9311(cab gene from 9311)。insilico分析表明:cab-9311与cab4基因同源性为99%,编码的蛋白含有244个氨基酸,与cab4基因编码的蛋白同源性为98%。蛋白分子质量为26.9kD,理论等电点为6.52。第54位～第216位氨基酸是一个典型的叶绿素a/b结合蛋白功能域(chlorophyll a/bbinding domain)。跨膜分析和蛋白质三级预测显示,该蛋白在C端有一个典型的跨膜区。亚细胞定位分析表明该蛋白定位于叶绿体,是一个叶绿体内囊体膜上的锚定蛋白。更多还原

【Abstract】 With the primer designed according to the Nipponbare cab4 gene sequence(GenBank:AK104499.1),a full-length cDNA gene encoding the light-harvesting chlorophyll a/b-binding protein in rice 9311 was cloned by RT-PCR,and it was named cab-9311(cab gene from 9311).In silico analysis shows that the cab-9311 cDNA sequence has 99% homology to the cab4 cDNA sequence.Cab-9311 encodes a protein of 244 amino acids which has 98% homology to the protein encoded by cab4 gene.The molecular weight of that protein is about 26.9 kD and its isoelectric point is 6.52.The amino acid residues 54~216 of that protein is a chlorophyll a/b binding domain.The analysis of transmembrane protein and the prediction of protein tertiary structure show a typical transmembrane domain is located in the C-terminus of that protein.The prediction of the subcellular location shows that protein is located in the chloroplast.All the results give a clue that the protein encoded by cab-9311 is embedded in the thylakoid membranes.更多还原