【摘要】 目的研究类风湿关节炎(RA)患者外周血中Th1、Th17与调节性T细胞(Treg细胞)的表达情况并与病情活动性进行相关性分析,探讨Th17、Th1与Treg细胞在RA的发病机制中作用及其临床意义。方法选择40例RA患者,根据疾病活动性评分(DAS28)评分进行分组;采用流式细胞术检测RA患者和20例健康对照者外周血中Th17、Th1及Treg细胞的比例;并分析各细胞与DAS28的相关性。结果 RA患者PBMCs中Th17细胞比例高于对照组(P<0.05),且RA活动组Th17细胞的比例高于稳定组(P<0.05);RA患者Treg细胞显著低于对照组(P<0.05);RA组和健康对照组Th1细胞水平无显著差异(P>0.05);Th17细胞数与RA疾病活动指标呈正相关。结论 Th1、Th17和Treg细胞在RA患者外周血中有不同的表达,提示Th17、Th1及Treg细胞在RA发病过程中发挥不同的作用。更多还原

【Abstract】 Objective To investigate the changes of Th17、Th1 and Treg in peripheral blood in patients with Rheumatoid arthritis(RA)and with disease activity the correlation analysis,and to discuss the role of Th17,Th1 and Treg cells in the pathogenesis of RA.Methods Forty patients with RA were recruited,grouping according to RA disease activity score(DAS28).Flow cytometric assay were used to analyse the percentages of Th17、Th1 and Treg cells in 40 cases of RA patients and 20 normal controls,and with disease activity correlation analysis.Results The percent of Th17 cells in patients with RA was significantly higher than that of normal controls(P<0.05),Th17 cells in the active RA group was higher than that of inactive group(P<0.05);The percent of Treg cells in patients with RA was significantly lower than that of normal controls(P<0.05);the levels of Th1 cells in RA patients and controls had no difference(P>0.05);The proportion of Th17 positively correlated with DAS28.Conclusion Th1,Th17 and Treg cells in peripheral blood of RA patients,suggest Th17,Th1 and Treg cells play different role in RA pathogenesis process.更多还原