【摘要】 目的建立胶体金免疫法(CGIA)对手足口病(HFMD)分型的检测方法。方法用双抗体夹心检测原理建立CGIA对HFMD分型检测方法 ,分别检测136例HFMD患儿和30例健康儿童粪便中柯萨奇病毒A16型(CA16)和肠病毒71型(EV71),以逆转录聚合酶链反应(RT-PCR)为金标准,评价CGIA检测性能。结果 CGIA与RT-PCR检测136例HFMD患儿粪便EV71阳性率分别为27.2%和28.6%;CGIA与RT-PCR检测136例手足口病患儿粪便CA16阳性率分别为33.8%和35.3%;以RT-PCR为金标准,CGIA检测手足口病患儿粪便EV71的敏感性89.7%,特异性89.7%,阳性预测值94.5%,阴性预测值95.9%,准确度95.5%;CGIA检测手足口病患儿粪便CA16的敏感性85.4%,特异性94.3%,阳性预测值89.1%,阴性预测值92.2%,准确度91.1%。结论 CGIA法操作简单、方便、快速,特异性和敏感性好,为HFMD病原学早期分型诊断提供了一个有效的检测手段。更多还原

【Abstract】 Objective To establish colloidal gold immunoassay(CGIA) for the classification of pathogens causing hand,foot and mouth disease(HFMD).Methods Double antibody sandwich detection principle was used to build CGIA classification test methods for HFMD.Coxsckievirus A16 type(CA16) and enterovirus71(EV71) were detected in feces samples from 136 cases of HFMD patients and 30 cases of healthy children.Reverse transcription-polymerase chain reaction(RT-PCR) was performed as gold standard method to evaluate the detection performance of CGIA.Results Positive rates of EV71 in 136 cases of HFMD patients,detected by CGIA and RT-PCR were 27.2% and 28.6%,respectively,and those of CA16 were 33.8% and 35.3%.Taking RT-PCR as gold standard method,the sensitivity,specificity,positive predictive values,negative predictive values and accuracy rate of CGIA to detect EV71 in HFMD patients were 89.7%,89.7%,94.5%,95.9% and 95.5%,respectively,and those of CGIA to detect CA16 in HFMD patients were 85.4%,94.3%,89.1%,92.2% and 91.1%,respectively.Conclusion The method of CGIA could be simple,convenient and fast,with fine specificity and sensitivity.The establishment of this method could provide an effective means for early etiology classification diagnosis of HFMD.更多还原