【摘要】 目的:观察安脑丸对大鼠脑出血后OX42、脑源性神经营养因子(BDNF)及突触素(SYN)表达的影响。方法:SD大鼠190只随机分为正常组10只,假手术组、模型组、安脑丸组各60只;后3组又随机分为造模后12h、1d、2d、4d、7d、10d共6个时间点,各10只。模型组制作脑出血模型,安脑丸组加用安脑丸灌胃。于各时间点采用免疫组化法及western blot法检测OX42、BDNF及SYN表达情况。结果:模型组及安脑丸组OX42及BDNF阳性细胞数及SYN、BDNF蛋白表达显著高于正常组及假手术组(P<0.01),安脑丸组OX42阳性细胞数显著少于模型组(P<0.01),BDNF阳性细胞数及SYN、BDNF蛋白表达显著高于模型组(P<0.01)。结论:小胶质细胞的激活、BDNF及SYN的表达与脑出血密切相关,安脑丸可显著减少脑出血后小胶质细胞的活化,增加BDNF及SYN的表达。更多还原

【Abstract】 Objective: To study the effects of An Nao Wan(ANW) on the expression of OX42,BDNF and SYN in rats after intracerebral hemorrhage.Methods: One hundred and ninety SD rats were randomly divided into normal control group(n=10),sham operated group,model group and ANW treatment group(n=60 respectively).Experimental intracerebral haematoma was made by injecting Ⅶ type collagenase into the right globus pallidus of rats in the model group and the ANW treatment group.The rats in the ANW treatment group were treated by ANW.Immunohistochemical staining and western blot analysis were employed to detect the expression of OX-42,BDNF and SYN at 12 h,1 d,2 d,4 d,7 d or 10 d after the operation.Results: The numbers of OX42 and BDNF positive cells and the expression of BDNF and SYN protein in the model group and the ANW treatment group were increased significantly when compared with those in the normal control group and the sham operated group(P<0.01).Compared with those in the model group,the number of OX42 positive cells was decreased significantly while the number of BDNF positive cells and the expression of BDNF and SYN protein were increased significantly in the ANW treatment group(P<0.01).Conclusion: The activation of microglia and the expression of BDNF and SYN were involved in the pathological process of intracerebral hemorrhage.ANW treatment could inhibit the activation of microglia and increase the expression of BDNF and SYN protein after intracerebral hemorrhage.更多还原