Objective: To optimize extraction process of BiQing suppository.Method: Extraction process was optimized with orthogonal design by taking water as extraction solvent,drug-liquid ratio,extracting time and times as the indexes,the extraction rate and content of berberine hydrochloride as evaluating indicators.Result: the optimal extraction was using ten folds amount of water,reflux extraction twice,once 1.5 hours.Conclusion: The extraction process is stable and could be used to the production of BiQingShuang.