【摘要】 目的:探讨5-氨基乙酰丙酸（5-ALA）光动力学疗法（photodynamictherapy,PDT）对体外培养人卵巢癌SKOV3和AO细胞的杀伤作用。方法:于体外培养的SKOV3和AO细胞分别加入不同浓度的5-ALA（0.1、0.5、1.0、2.5、12.5 mmol/L）孵育4 h,以波长为630 nm的半导体激光进行照射,照射能量密度分别为0.1、0.5、2.5、12.5 J/cm²。用四唑盐（MTT）比色法测定细胞存活率,并与单纯药物对照组（不同浓度5-ALA孵育、无光照）、单纯光照组（无5-ALA孵育、不同能量密度光照）与阴性对照组（无药物孵育、无光照）进行比较。计算5-ALA-PDT对SKOV3和AO细胞的半数杀伤浓度(ICs₅₀)。结果:5-ALA-PDT处理后两种卵巢癌细胞存活率均明显下降（P<0.05）,提示5-ALA-PDT对体外培养的人卵巢癌SKOV3和AO细胞均有杀伤作用,其杀伤作用随5-ALA的浓度和激光能量密度增加而增加,当5-ALA浓度超过2.5mmol/L或照射能量密度超过2.5 J/cm²时细胞存活率变化不明显。单纯药物对照组、单纯光照组的细胞存活率与阴性对照组相比差异无显著性（P>0.05）。5-ALA-PDT对两种卵巢癌细胞杀伤效应强弱不同,对SKOV3及AO细胞的半抑制浓度(IC₅₀)分别为0.34mmol/L和2.50 mmol/L,两者差异有显著性（P<0.05）。结论:5-ALA-PDT可有效杀伤体外培养的人卵巢癌细胞SKOV3和AO细胞,且对两种细胞的杀伤作用有差异,对SKOV3细胞株的杀伤效应要强于AO细胞株。更多还原

【Abstract】 Objective:To investigate the effect of 5-aminolevulinic acid（5-ALA）-photodynamic therapy（PDT） on ovarian carcinoma cell lines SKOV3 and AO in vitro.Methods:SKOV3 and AO cells were incubated in a medium containing different concentrations of 5-ALA（0.1,0.5,1.0,2.5,12.5 mmol/L） for 4 hours,and they were exposed to different light doses（0.1,0.5,2.5 or 12.5 J/cm²） of semiconductor laser at 630 nm.The survival rate of SKOV3 and AO cells was analyzed by MTT assay,and compared to that of the simple drug-control group （which was incubated with various concentrations of 5-ALA without exposure to laser）,simple laser-control group （which was illuminated with different doses of laser exposure without drug） and negative control group.The half inhibitory concentration(IC₅₀) of PDT on AO and SKOV3 cells was calculated.Results:After 5-ALA-PDT treatment, the survival rates of both ovarian carcinoma cell lines were significantly lowered（P<0.05）,suggesting that 5-ALA-PDT could kill ovarian carcinoma cell lines SKOV3 and AO in vitro,and the killing effects showed concentration -dependant on 5-ALA and energy density-dependant on laser,until the concentration of 5-ALA reached 2.5 mmol/L or the light doses reached 2.5 J/cm².Compared with the negative control group,the changes in cell survival rates of simple drug-control group and simple laser-control group were not significant（P>0.05）.The half inhibitory concentration of PDT on AO and SKOV3 cells was 0.34 mmol/L and 2.50 mmol/L respectively,the difference was statistically significant（P<0.05）.Conclusions:5-ALA-PDT may effectively kill the SKOV3 and AO cells in vitro,and the effect on SKOV3 cells is stronger than on AO cells.更多还原