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HPLC测定黄芪药材芒柄花素

Determination of Formononetin in Astragalus membranaceus(Fisch.)Bge.by HPLC

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【作者】 黄志勤程齐来李洪亮

【Author】 HUANG Zhi-Qin CHENG Qi-Lai LI Hong-Liang(School of Pharmacy,Gannan Medical University,Ganzhou,Jiangxi 341000,P.R.China)

【机构】 赣南医学院药学院

【摘要】 建立黄芪药材质量标准,研究不同产地黄芪药材的质量。采用高效液相色谱法(HPLC),对我国7个省68个黄芪样品进行了芒柄花素成分的含量测定,以芒柄花素含量作为衡量优劣指标。7个省68个黄芪药材样品检测结果表明,不同产地黄芪药材芒柄花素成分的含量差异较大,其中:山西>内蒙>甘肃>辽宁;不同品种黄芪药材芒柄花素成分的含量差异较大,其中:栽培品种<野生品种;生长年限不同,芒柄花素成分的含量也不同,其中:二年生>四年生>五年生>三年生。采用HPLC进行黄芪药材的质量评价切实可行,为制药企业选用黄芪药材提供科学、可靠的依据。

【Abstract】 The quality level of Astragalus membranaceus(Fisch.)Bge.was established,and the qualities of Astragalus membranaceus(Fisch.) Bge.from different producing areas were investigated.The contents of formononetin componential in 68 Astragalus membranaceus(Fisch.) Bge.from 7 provinces in China were determined by high performance liquid chromatography(HPLC) with the contents of formononetin as measure quality index.The detection results of 68 Astragalus membranaceus(Fisch.)Bge.from 7 provinces showed the differences of formononetin componential contents were large in Astragalus membranaceus(Fisch.)Bge.from different producing areas,and contents ordered as follows:Shanxi>Inner Mongolia>Gansu>Liaoning.The differences of formononetin componential contents were big in different variety of Astragalus membranaceus(Fisch.)Bge.,but the contents of cultivar variety were less than wild variety.The contents of formononetin componential were different due to different growing time,and contents of growing time lawed the order as follow:two years>four years>five years>three years.The quality evaluation of Astragalus membranaceus(Fisch.)Bge.by HPLC was available,and can provide the scientific and confident judgment to pharmacy factory for the selection of raw material of Astragalus membranaceus(Fisch.)Bge.

  • 【文献出处】 光谱实验室 ,Chinese Journal of Spectroscopy Laboratory , 编辑部邮箱 ,2012年05期
  • 【分类号】R284.1
  • 【被引频次】3
  • 【下载频次】226
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