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HPLC测定羟丙基环糊精/蒿甲醚包合物中蒿甲醚
Determination of Artemether in Inclusion Compound of ATM-HP-β-Cyclodextrin by HPLC
【摘要】 建立用HPLC测定蒿甲醚-羟丙基-β-环糊精包合物中蒿甲醚含量的方法。色谱柱为Lichrospher C18柱,乙腈-水(60:40,V/V)为流动相,检测波长210nm。包合物中蒿甲醚的含量在5—35μg范围内与峰面积呈线性关系(r=0.9998)。平均加样回收率为97.87%,RSD为0.66%(n=5)。
【Abstract】 The method for the determination of the content of artemether in artemether-hydroxypropyl-β-cyclodextrin(HP-β-CD) by HPLC was established.The samples were separated by chromatographic column Lichrospher C18 with mobile phase consisted of acetonitrile-water(60∶40,V/V),and detection wavelength was 210nm,there was the linear relation in the range of 5—35μg(r=0.9998).The average additional standard recoveries was 97.87% with RSD of 0.66%(n=5).
【关键词】 蒿甲醚;
蒿甲醚-羟丙基-β-环糊精包合物;
高效液相色谱法;
【Key words】 Atemther; Inclusion Compound of ATM-HP-β-CD; HPLC;
【Key words】 Atemther; Inclusion Compound of ATM-HP-β-CD; HPLC;
【基金】 国家自然科学基金NNSFC(21062009);云南省应用基础研究基金(2009ZC45M)
- 【文献出处】 光谱实验室 ,Chinese Journal of Spectroscopy Laboratory , 编辑部邮箱 ,2012年03期
- 【分类号】O657.72;R284
- 【被引频次】1
- 【下载频次】179