【摘要】 目的:观察糖尿病小鼠视网膜血管内白细胞淤滞和细胞间黏附因子-1(ICAM-1)在视网膜的表达。方法:选取C57型小鼠45只,随机分为2组:糖尿病组22只、正常组23只。10wk后取视网膜,荧光显微镜计数小鼠全视网膜微血管内淤滞的白细胞数目及其含有ICAM-1表达的荧光小球数目,免疫蛋白印迹法检测视网膜血管内皮生长因子(VEGF)和ICAM-1的表达。结果:10wk后,糖尿病小鼠视网膜血管内白细胞数目及含有ICAM-1表达的荧光小球数目明显高于正常对照组(P<0.01),其视网膜内VEGF和ICAM-1明显增加,有统计学意义(P<0.05)。结论:视网膜血管内白细胞淤滞与糖尿病视网膜病变(DR)早期的视网膜毛细血管无灌注有关。更多还原

【Abstract】 AIM:To investigate the leukostasis and expression of ICAM-1 in 10 weeks diabetic mice retina.METHODS:Forty-five C57 mice(20-25g) were randomly divided into two groups.There were twenty-two mice in diabetic group and twenty-three mice in normal group.The leukostasis in diabetic retinopathy was counted and the expression of VEGF and ICAM-1 was studied by Western Blot.RESULTS:Ten weeks postoperatively,the number of leukocyte was significantly high in diabetic retinopathy(P<0.01).The expression of VEGF and ICAM-1 was significantly increased in diabetic group(P<0.05).CONCLUSION:The no perfusion of retinal blood capillary is related to leukostasis in early diabetic retinopathy.更多还原