【摘要】 为获得具有高解磷活性,可用于微生物肥料研制和生产的无机磷细菌菌株资源,采用平板溶磷圈法,从秦岭山区植物根际土壤样品中筛选获得3株高效无机磷细菌,命名为NO.9、NO.15、NO.17,根据钼锑抗比色法,培养5 d后,检测菌株发酵液,确定3株细菌的可溶性磷含量分别为98.12、80.37 mg/L和104.91 mg/L,微生物量磷含量分别为62.85、53.61、36.59 mg/L。生理生化特征、Biolog和16S rDNA鉴定结果表明,3株细菌均属于革兰氏阴性非肠道菌,NO.9菌株为Inquilinus ginsengisoli,NO.15菌株为醋酸钙不动杆菌(Acinetobacter calcoaceticus),NO.17菌株为荧光假单胞菌(Pseudomonas fluorescens)。更多还原

【Abstract】 A study was conducted to isolate effective inorganic phosphorus-solubilizing bacteria for potential research and production of biofertilizer.Three strains,named as NO.9,NO15 and NO.17,are isolated from soil samples collected from Qinling Mountains by their dissolving phosphorus circle on PKO Agar media.After 5 days incubation,through Mo-Sb colorimetry,the soluble phosphorus content in liquid media is 98.12 mg/L,80.37 mg/L and 104.91 mg/L respectively,and the content of microbial biomass phosphorus is 62.85 mg/L,53.61 mg/L and 36.59 mg/L.Analysed by physiology and biochemistry experiments,Biolog and 16S rDNA sequencing reflects that all of the three strains are gram-negative intestinal bacteria.and NO.9 strain is identified as Inquilinus ginsengisoli,NO.15 strain as Acinetobacter calcoaceticus and NO.17 strain as Pseudomonas fluorescens.更多还原