【摘要】 采用盆栽试验法研究了在干旱及盐双胁迫下内生固氮菌接种对多枝柽柳实生苗生理特性的影响。结果表明:接菌植株较未接菌植株SOD酶活性在水盐胁迫条件下平均提高68.3%,在非胁迫条件下平均提高52.0%,随着胁迫时间延长和胁迫强度增加,SOD酶活性维持在275.82～561.49 u/g。POD酶活性在水盐胁迫条件下呈现先上升后下降再急剧升高的波动特点,活性提高主要发生在胁迫的中期和后期。内生固氮菌接种还能有效降低水盐胁迫条件下枝叶细胞质膜相对透性,减缓根系活力的下降幅度,维持生理活动的正常运行。接种内生固氮菌提高了多枝柽柳植株在水盐胁迫条件下的适应调节能力和根系活力,保护了细胞膜的功能和结构,减弱了逆境对植株细胞膜的伤害程度,增强了植物对逆境的耐受能力。更多还原

【Abstract】 Endophytic diazotrophic bacteria are the microorganisms that colonizing various living plants tissues and establishing symbiosis relationship with the host,these bacteria are believed having features such as nitrogen-fixing,plant growth promoting,enhancing nutrient absorption,stress resistance of host plant.In this paper,the effects of inoculation endophytic diazotrophic bacteria on physiological characters of Tamarix ramosissima Ledeb.seedlings were studied using pot experiments under salt-drought stress,and the resistance mechanisms were discussed.The experimental design included four levels of water stress(75%,60%,40% and 20% of soil relative water content) and four levels of salt stress(0 g/L,4 g/L,6 g/L and 8 g/L of soil salt content),compared with plant tolerance to stress on inoculated and uninoculated endophytic diazotrophic bacteria strain,the results showed that the activities of superoxide dismutase(SOD) increased 68.3%,52.0% under salt-drought stress and non-stress,respecively,in addition,the activities of SOD can maintain in 275.82-561.49 U·g-1 with prolong the period and intensive of stress.When salt-drought stress lasting 60 days,the activities of SOD can maintain high level under 0 g/L and 4 g/L salt in 60% water treatment.The activities of peroxidase(POD) showed increase firstly,then decrease and increase rapidly again,which mainly enhanced in middle and late stage of salt-drought stress and showed a greater difference in growth period,through the stress period,the activities of peroxidase(POD) can maintain high level under 0 g/L and 8 g/L salt concention in 60% water treatment at 60 days,moreover,various protective enzyme may play different role at different stages of stress.At the same time,inoculation endophytic diazotrophic bacteria also can efficiently reduce the cell membrane permeability and slow down the root activity dropping,so to keep normal physiological activities.After 20 and 40 days salt-drought stress,the cell membrane permeability of all treatment maintained low level,while the root maintained vitality steadily at 20 days stress.In conclution,under salt-drought stress,inoculation endophytic diazotrophic bacteria can enhance the host’s ability to adapt to stress environment,effectively heighten the root vitality,protect structure and weaken injury of plant cell membrane,therefore increase plant adversity resistance.更多还原