【摘要】 目的探讨膀胱尿路上皮癌中肿瘤转移抑制基因TIP30/CC3基因的表达及意义。方法收集武汉大学人民医院病理科2000-2006年有完整临床和病理资料的膀胱尿路上皮癌存档蜡块50例和5例癌旁组织,采用免疫组织化学S-P法检测50例膀胱尿路上皮癌和5例癌旁组织中TIP30/CC3基因的表达水平。并分析TIP30/CC3基因与膀胱尿路上皮癌临床和病理特征的关系。采用HPIAS-1000高清晰度彩色病理图文报告管理系统,对TIP30/CC3基因的表达进行定量分析,并用SPSS13.0软件对各组免疫组织化学反应阳性颗粒的平均光密度、阳性面积率做单因素方差分析和SNK(q)检验。结果 (1)TIP30/CC3基因在膀胱尿路上皮癌中呈低表达,癌旁组织中呈高表达。膀胱尿路上皮癌与癌旁组织相比,差异有显著性(P<0.05);(2)TIP30/CC3的表达与患者年龄、性别之间的差异均无显著性(P>0.05)。但与肿瘤浸润深度、分化程度和临床UICC分期之间的差异均有显著性(P<0.05)。结论抑癌基因TIP30/CC3基因在膀胱尿路上皮癌中的低表达可能促进了膀胱尿路上皮癌的发生和发展。更多还原

【Abstract】 Objective To investigate the expression and significance of Tip30/CC3 in bladder urothelial cancer tissues.Methods Parafflin-embeded specimens of 50 cases of bladder urothelial cancer,and 5 cases of normal pericancerous tissues,which were all filed in full pathological and clinical data,were collected from the pathology department of the People Hospital of Wuhan University between 2000 and 2006.The expression of Tip30/CC3 was detected by immunohistochemical S-P method,and the relationship between Tip30/CC3 and the clinical and pathologic features of bladder urothelial carcinoma was analyzed.Quantitative analysis of Tip30/CC3 was conducted by high-definition pathological graphics context report system(HPIAS-1000).One-way analysis of variance and SNK(q) tests was used to analyze the mean density and the positive area rate of the immunohistochemical results.All data were processed by SPSS13.0.Results The expression of Tip30/CC3 in pericancerous tissues significantly higher than that was in bladder urothelial cancer(P<0.05).Conclusion The low expression of the tumor-suppressor gene TIP30 / CC3 may promote the occurrence and development of bladder urothelial carcinoma.更多还原