【摘要】 应用PCR扩增猪源沙门氏菌GyrA基因的喹诺酮耐药决定区,并对扩增产物进行SSCP分析,比较SSCP图谱与耐药性之间的相关性。同时对GyrA基因扩增产物进行测序,分析氨基酸的变异情况。结果显示,猪源沙门菌GyrA基因的喹诺酮耐药决定区PCR扩增产物的大小为317 bp,敏感菌株与标准菌株SSCP图谱相一致,耐药菌株与标准菌株的SSCP图谱差异明显,GyrA基因QRDR的突变位于第83和87位氨基酸位点。通过SSCP可早期、准确、快速地检测出猪源沙门氏菌是否对氟喹诺酮耐药。更多还原

【Abstract】 The Quinolone resistance determining regions(QRDR) of GyrA genes of Salmonella suipestifer were amplified by PCR and PCR products were analysed by single-strand conformation polymorphism(SSCP) to discuss the relativity between resistance and SSCP map.PCR products were directly sequenced for detection of mutation in gene.The results showed that the number of QRDR of GyrA genes was 317 bp.The SSCP map of sensitive bacteria strains was according with standard strain,but it was distinctly different between resistant bacteria and standard strains.The mutation sites of GyrA were found in codon 83 and codon 87.The SSCP technique could accurately,quickly and early detected the resistance of Salmonella suipestifer to quinolone.更多还原