【摘要】 对克隆获得的转录因子NF-κB/Rel家族成员Relish基因在斑节对虾发育阶段的表达特征进行分析。所获的PmRelish是Relish基因的亚型之一,与凡纳滨对虾LvRelish高度相似,结构域组成相同,其中RHD、IPT和DD结构域均具有100%的同源性,ANK结构域的相似性也高达97.7%。NF-κB/Rel家族进化树分析显示,PmRelish与对虾、昆虫等节肢动物的Relish聚为一支,并与P105(NF-κB1)和P100(NF-κB2)最为相近。PmRelish的表达在4个幼体发育时期都存在显著性差异,其中糠虾阶段表达量最高。PmRelish在不同发育阶段的精巢和卵巢中的表达特征相似,即未成熟期的表达相对较低,其中PmRelish在10～12 cm体长雄虾精巢中表达量最高,卵巢中的表达则在成熟期(V期)最高,为卵原细胞期的14.3倍。更多还原

【Abstract】 The paper obtained a transcription factor Relish from Penaeus monodon,a member of the NF-κB/Rel family,and analyzed its expression profile at the different developmental stages.PmRelish,a subtype of the Relish gene,is highly similar to LvRelish from Litopenaeus vannamei,with the same composition of domains.Their RHD,IPT and DD domain have 100% homology,and the similarity of the ANK domain is up to 97.7%.Phylogenetic tree of NF-κB/Rel family shows that PmRelish is clustered with other arthropods Relish including other shrimps and insects,and near to P105(NF-κB1) and P100(NF-κB2).The expression of PmRelish among four periods of larval development has a significant difference,with the highest expression level in mysis stage.The expression profile of PmRelish at different developmental stages in testis is similar to that in ovary,with a low expression level at immature phases.The expression level of PmRelish is highest in testis of male tiger shrimp with body length of 10～12 cm,and is highest in mature stage ovary of female tiger shrimp with a 14.3 folds higher than that in oogonium stage.更多还原