【摘要】 制备高活性壳寡糖并对其生物活性进行研究。采用专一性壳聚糖酶酶解壳聚糖制备壳寡糖,乙酰丙酮法测定壳寡糖的数均分子量,用不同剂量壳寡糖灌喂小鼠,探讨壳寡糖对小鼠免疫功能的影响以及对小鼠肝脏的保护作用。结果表明,所得壳寡糖的数均分子量为1 246.38,聚合度为4~6;该壳寡糖对小鼠免疫器官具有明显的保护和促进生长作用,显著提高了小鼠的抗疲劳能力以及抗菌活力,对小鼠肝脏具有显著的保护作用。可见,专一性壳聚糖酶酶解所得聚合度4~6的壳寡糖具有较高的生物活性,壳寡糖在保健食品开发及医药等领域的应用前景广阔。更多还原

【Abstract】 To prepare high activity chito-oligosaccharide and to study its biological activity,the number average molecular mass of chito-oligosaccharide which prepared by degradation with specific chitosanase was determined with acetylacetone method.Mice were administered with chito-oligosaccharide with different doses,to discuss the effects of chito-oligosaccharide on immune function in mice and the protective effect on liver.The results showed that the number-average molecular of chito-oligosaccharides was 1 246.38,the degree of polymerization was 4 to 6;the chito-oligosaccharide had a significant role on the protection and promotion of mice’ immune organ,it also significantly increased the mice’s resistance to fatigue and anti-bacterial vitality,and had a significant protective effect on mice liver.The chito-oligosaccharide of degree of polymerization in 4~6 which prepared by degradation with specific chitosanase contains high biological activity,so chito-oligosaccharides look a promising future for application in the health food and medical field.更多还原