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小麦avenin-like type-B基因启动子的分离及表达载体的构建

Isolation of Avenin-like type-B gene promoter from wheat and construction of expression vector

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【Author】 SONG Fei1,CHEN Ling1,2,SUN Yang-liu1,WANG Fei-fei1,WANG Yue-sheng1, YANG Guang-xiao1,HE Guang-yuan1 (1.China-UK HUST-RRes Genetic Engineering and Genomics Joint Laboratory/The Genetic Engineering International Cooperation Base of Chinese Ministry of Science and Technology/Key Laboratory of Molecular Biophysics of Chinese Ministry of Education, College of Life Science and Technology,Huazhong University of Science & Technology(HUST),Wuhan 430074,China; 2.Institute of Food Crops,Hubei Academy of Agricultural Sciences,Wuhan 430064,China)

【机构】华中科技大学生命科学与技术学院中英HUST-Rres基因工程和基因组学联合实验室/科技部国际科技合作基地(基因工程)/教育部分子生物物理重点实验室；湖北省农科院粮食作物研究所；

【摘要】根据已知的小麦ALP type-B基因序列,采用反向PCR技术,从小麦品种鄂恩1号(En1)中克隆得到了ALP type-B基因的上游启动子序列1664 bp。通过PLACE和plantCARE等数据库对所克隆的启动子进行生物信息学分析,发现该启动子除具备启动子的基本元件如TATA-box、CAAT-box等外,还含有胚乳特异性表达的特有元件如Endosperm motif、GCN4-like motif(GLM)、RY motif和G-box等。用ALP type-B启动子置换质粒pBI121上的35S启动子,将其与gus基因连接构建植物表达载体。更多还原

【Abstract】 The study successfully cloned a 1 664 bp upsteam sequence of ALP from wheat En1 using Inverse-PCR(IPCR),based on the sequence of ALP type-B gene.Putative fuctional promoter elements were analyzed by the PLACE datebase and PlantCARE datebase.The results showed that the upsteam sequence contained the basic elements such as CAAT-box and TATA-box.Endosperm motif,GCN4-like motif(GLM),RY motif and G-box were also detected as endosperm-specific elements.Expression vector harboring the promoter fused with gus gene was constructed by replacing the 35S promoter in pBI121.更多还原

【关键词】小麦；胚乳特异性基因；启动子；植物表达载体；
【Key words】 wheat； endosperm-specific gene； promoter； plant expression vector；

【基金】国家转基因重大专项(2011ZX08010004-004)

【文献出处】广东农业科学 ,Guangdong Agricultural Sciences , 编辑部邮箱 ,2012年04期

【分类号】S512.1
【被引频次】1
【下载频次】100

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