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高效液相色谱法快速测定红酒中的白藜芦醇含量
Rapid Detection of Resveratrol in Red Wine by HPLC
【摘要】 文章建立了高效液相色谱法测定红酒中白藜芦醇的方法,色谱柱为Agilent ZORBAX Eclipse XDB C18(4.6 mm×250 mm,5μm),以乙腈-水(35∶65,水相中含0.1%冰乙酸)为流动相,流速1 mL/min,进样量20μL,室温,检测波长300 nm,外标法定量,该方法相对标准偏差为1.18%~1.99%,线性相关系数R2为0.999,检测限为2μg/mL。在上述条件下测定了随机抽取的部分市售红酒样品的白藜芦醇含量力。
【Abstract】 The paper focused on the conditions for resveratrol detection by HPLC.The detection conditions were shown as following:the column was Agilent ZORBAX Eclipse XDB C18(4.6 mm×250 mm,5 μm) column,acetonitrile-water(35∶65,with 0.1 % acetic acid in water) as mobile phase,flow rate was 1 mL/min and the injection volume was 20 μL,the column temperature was at room temperature and detection wavelength was 300 nm.The relative standard deviation ranges from 1.18 % to 1.99 %,linear correlation coefficient R2 was of 0.999 and the detection limit was 2 μg/mL.The resveratrol concentration of commercially available red wine samples was detected by this HPLC method.
- 【文献出处】 广东化工 ,Guangdong Chemical Industry , 编辑部邮箱 ,2012年07期
- 【分类号】R284.1
- 【被引频次】2
- 【下载频次】241