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高效液相色谱法快速测定红酒中的白藜芦醇含量

Rapid Detection of Resveratrol in Red Wine by HPLC

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【作者】 赵丽青方佳茂肖向东陈伟滨张帆

【Author】 Zhao Liqing1,Fang Jiamao2,Xiao Xiangdong3,Chen Weibin2,Zhang Fan1(1.Department of Food Science and Engineering,College of Chemistry and Chemical Engineering,Shenzhen University,Shenzhen 518060;2.Guangdong Huanxi Biological Technology Co.,Ltd.,Puning 515343;3.School of Medicine,Shenzhen University,Shenzhen 518060,China)

【机构】 深圳大学化学与化工学院食品科学与工程系广东环西生物科技股份有限公司深圳大学医学院

【摘要】 文章建立了高效液相色谱法测定红酒中白藜芦醇的方法,色谱柱为Agilent ZORBAX Eclipse XDB C18(4.6 mm×250 mm,5μm),以乙腈-水(35∶65,水相中含0.1%冰乙酸)为流动相,流速1 mL/min,进样量20μL,室温,检测波长300 nm,外标法定量,该方法相对标准偏差为1.18%~1.99%,线性相关系数R2为0.999,检测限为2μg/mL。在上述条件下测定了随机抽取的部分市售红酒样品的白藜芦醇含量力。

【Abstract】 The paper focused on the conditions for resveratrol detection by HPLC.The detection conditions were shown as following:the column was Agilent ZORBAX Eclipse XDB C18(4.6 mm×250 mm,5 μm) column,acetonitrile-water(35∶65,with 0.1 % acetic acid in water) as mobile phase,flow rate was 1 mL/min and the injection volume was 20 μL,the column temperature was at room temperature and detection wavelength was 300 nm.The relative standard deviation ranges from 1.18 % to 1.99 %,linear correlation coefficient R2 was of 0.999 and the detection limit was 2 μg/mL.The resveratrol concentration of commercially available red wine samples was detected by this HPLC method.

【关键词】 白藜芦醇红酒高效液相色谱法
【Key words】 resveratrolred wineHPLC
【基金】 深圳大学基础研究科研项目(No.00035226)
  • 【文献出处】 广东化工 ,Guangdong Chemical Industry , 编辑部邮箱 ,2012年07期
  • 【分类号】R284.1
  • 【被引频次】2
  • 【下载频次】241
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