【摘要】 目的观察内毒素(LPS)预处理对内毒素血症鼠的作用并探讨其机制。方法将雄性Wistar大鼠72只随机分为生理盐水组(N组,n=24)、LPS对照组(L组,n=24)、LPS预处理组(P组,n=24),P组首先腹腔注射LPS,24 h后再经腹腔注射LPS,N组和L组在上述两时点均给予等容量生理盐水,72 h后L组和P组分别静脉注射LPS,N组给予等容量NS。各组分别取6只大鼠用于持续观察血流动力学改变,并于造模后2、4、6 h时分别另取6只大鼠采血测定肿瘤坏死因子-α(TNF-α)和一氧化氮(NO)含量,并于造模后6 h取动物心、肺、肝、肾、小肠等脏器组织,观察其形态学变化。结果 LPS预处理可明显逆转大鼠内毒素血症时的血流动力学如平均动脉压、心率和呼吸频率的改变:P组与L组比较差异有统计学意义,而与N组差异无统计学意义。L组大鼠血浆中TNF-α、NO的含量较N组、P组明显升高(P<0.05),但N组与P组间差异无统计学意义。HE染色发现LPS预处理可减轻大鼠内毒素血症时心、肺、肝、肾、小肠的病理变化。结论 LPS预处理可能通过减少TNF-α和NO的释放,对鼠LPS血症起到保护作用。更多还原

【Abstract】 Objective To investigate the effects and mechanism of lipopolysaccharide(LPS) pretreatment on endotoxemia in rats.Methods Male Wistar rats were randomly divided into three groups: saline group(N,n=24),LPS-treated group(L,n=24),and LPS-pretreated group(P,n=24).Intrapreitoneal injection of LPS was given to rats in Group P at baseline and 24th hour,while NS were given to rats in Group N and Group L instead.Intravenous injections of LPS were given to rats in Group L and P for construction of endotoxemia,while NS was given to Group N.The hemodynamics was observed constantly.TNF-α and NO levels were analyzed 2,4 and 6 hours after modeling.The lung,heart,liver,kidney and small intestine tissues were stained with hemotoxylin and eosin for observation.Results The pretreatment of LPS significantly reversed the endotoxemia-induced hemodynamic change(P<0.05).Significantly elevation of TNF-α and NO were revealed in Group L,when comparing with those in Group N,which were significantly attenuated by pretreatment of LPS in Group P(P<0.05).Moreover,significant alleviation in LPS-induced pathological impairment in heart,lung,liver,kidney and intestine were also observed in Group P.Conclusion Pretreatment of small dose of LPS protects rats from endotoxemia via reduction of TNF-α and NO.更多还原